Figure 2

Binding properties of M2e-specific scFv-msFc-γ2c antibodies. (A) Determination of affinity. The dissociation constants (Kd) of antibody binding to M2e-cons peptide in solution was determined by Friguet ELISA. (B) Binding to H5N1 Influenza A derived M2e. Antibody binding to the indicated RNAse-M2e conjugates was measured at different concentrations by ELISA. (C) Binding to cell surface M2. L929-M2#E9 cells, a clone of L929 cells expressing full-length M2 derived from mouse-adapted H1N1 Influenza A PR/8/34, were stained with the indicated antibodies and analyzed by FACS. Bold lines, 0.5 μg/ml; solid lines, 60 ng/ml; dotted lines, secondary antibody alone.