Figure 2
From: Interaction of influenza virus NS1 protein with growth arrest-specific protein 8
Protein-protein interaction domains. (A) Mapping of the NS1 domain interacting with Gas8. 293FT cells were transfected with pCMV-Tag 2B-Gas8 and one of its truncated derivatives: pCMV-myc-NS11-80, pCMV-myc-NS181-238, or pCMV-myc. After incubation for 36 h, cell lysates were IPed with anti-myc antibody and probed with anti-FLAG and anti-myc antibody. (B) Schematic representation of Gas8 proteins. Polypeptides corresponding to Gas81-478, Gas81-260, and Gas8260-478 are shown. The corresponding amino acids are indicated on the right. 293FT cells were transfected with pCMV-myc-NS1 and one of its truncated derivatives: pCMV-Tag 2B-Gas81-260, pCMV-Tag 2B-Gas8260-478, or pCMV-Tag 2B. Cell lysates were IPed with anti-myc antibody and probed with anti-FLAG and anti-myc antibody. +: protein-protein interaction; -: no protein-protein interaction.