Figure 1
From: Hepatitis C virus core protein induces apoptosis-like caspase independent cell death
Expression of different HCV-proteins in the UHCV and UC cells (A, B) and their induction of apoptotic nuclei (C, D): 2 × 106 cells (A, B) or 1 × 104, 2 × 104, and 3 × 104 cells/well (C, D) of each cell line were cultured for the indicated time in the presence or absence of tetracycline (Tet) to induce HCV-specific protein expression. A, B: Cellular proteins were resolved by SDS-PAGE and HCV proteins were detected by immunoblotting with an antiserum generated against the core (A) or NS3 protein (B) of HCV. C, D: Induction of apoptosis was assessed by flow cytometric analysis of propidium iodide staining of hypodiploid apoptotic nuclei. The mean values and standard deviation of triplicate cultures are shown.