The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

Table 2 Primers used to generate a serial of plasmids to validate the activity of the promoter

Primer	Sequence(5¹-3¹)	The sites opposite to the ORF of pp38[4]	Restriction enzyme sites	Fragment generated/bp
F_pp38	AAggtacc GAGCATCGCGAAAGAGAGA	-690~-671	Kpn I	322
R_pp38	GTgagctc TCGAGGCCACAAGAAATT	-393~-374	Sac I
F_{1.8 kb}	AAgagctc GAGCATCGCGAAAGAGAGA	-690~-671	Sac I	322
R_{1.8 kb}	GAggtacc TCGAGGCCACAAGAAATT	-393~-374	Kpn I
F(d)_pp38	TTTggtacc GTTCGCACCAGAGTCCA	-536~-519	Kpn I	168
R(d)_pp38	GAAgagctc GAGGCCACAAGAAATT	-393~-374	Sac I
F(d)_{1.8 kb}	AAgagctc GAGCATCGCGAAAGAGAGA	-690~-671	Sac I	161
R(>d)_{1.8 kb}	AAAggtacc GCCGAGGTGAGCCAATC	-552~-535	Kpn I

ISSN: 1743-422X