Figure 1
Schematic representation of HAV constructs containing insertions in the 2A-2B junction. Antibiotic resistance genes were cloned the 2A-2B junction of the HAV genome in pHAVvec9 [19]. This plasmid contains a polylinker coding for SalI, SnaBI, and KpnI restriction sites flanked by Gly hinges (grey color letters) and HAV 3Cpro cleavage site (arrows) engineered into 2A-2B junction of pT7HAV. The blasticidin resistance gene (Bsd) coding for blasticidin deaminase, green fluorescent protein (GFP)-Bsd fusion protein, and hygromycin resistance gene (Hyg) coding for hygromycin phosphotransferase were cloned into the SalI and KpnI sites in the polylinker of pHAVvec9, and the resulting plasmids were termed pHAVvec9-Bsd, pHAVvec9-GFP-Bsd, and pHAVvec9-Hyg, respectively.