Figure 8

Tissue distribution of the UL31 antigen in various organs of DEV-infected ducks. Mock- and infected ducks were sacrificed at 4 d post-infection. Each organ was from the ducks and fixed in 4% formaldehyde solution. After fixation, the tissue samples were dehydrated and embedded in paraffin. Then the tissue sections were made at 4 μm and stained with an indirect immunofluorescent technique. Labels on the left side of the images indicate organs. Negative control is shown in the left column, and the staining methods are indicated above the top horizontal row. (Images were acquired by using 40× objective).