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Figure 3 | Virology Journal

Figure 3

From: Comparative functional analysis of Jembrana disease virus Tat protein on lentivirus long terminal repeat promoters: evidence for flexibility at its N-terminus

Figure 3

Requirement for P-TEFb in LTR activation by jTat. (A) Competitive inhibition assays. HeLa cells (upper graphs) were co-transfected with 50 ng hTat, 25 ng pHIV-LTR-luc, 50 ng pCMV-lacZ and indicated amounts of hTat47 (left) or jTat67 (right). Similar experiments were performed in BL12 cells using pjTat and pJDV-LTR-luc instead of hTat and pHIV-LTR-luc. hTat47; hTat residues 1-47. jTat67; jTat residues 1-67. (B) HIV LTR activation by jTat in HeLa cells with depleted hCycT1 or CDK9. HeLa cells were transfected with indicated amounts of antisense plasmids against hCycT1 (rT1) or CDK9 (rCDK9) and tested for jTat activity on the HIV LTR reporter. Western analysis shows dose-dependent effect of antisense plasmids on expression of exogenous Flag-hCycT1 or Flag-CDK9 (see Methods for experimental protocol). Total protein levels in the western analysis were equivalent.

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Virology Journal

ISSN: 1743-422X