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Figure 3 | Virology Journal

Figure 3

From: Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli

Figure 3

Purification of recombinant proteins expressed in E. coli and immunodetection. Purification was performed as described in the text. Panel A, purified p24 (Lane 1) and purified RTB/p24 (Lane 2). Arrows indicate the expected proteins. Panel B, western blot of p24 purified protein, immunodetected with anti-p24 antibody at dilution of 1/1000. Panel C, western blot of purified RTB/p24 chimeric protein using three different antibodies, anti-p24 (1/1000), anti-His (1/1000), and anti-Ricin (1/3000). M, molecular weight markers in kDa. At the bottom a diagram of the two constructs is included.

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Virology Journal

ISSN: 1743-422X