Figure 2

UL56 localizes to cytoplasmic vesicular structures in transiently UL56-expressing cells. Transiently UL56-expressing (A, C) or UL56-non-expressing cells (B) were immunostained for UL56 (shown in green) and/or marker proteins for Golgi, trans-Golgi network (TGN) or endosomes (shown in red). (A) UL56 is distributed the cytoplasm in a vesicular pattern with accumulation in the perinuclear region. The serial confocal sections of UL56-expressing cells in the x-y plane with x-z (top panels) and y-z (right panels) projections are shown. X-z sections are shown with the apical side down; and y-z sections are shown with the apical side to the left. Yellow lines indicate the z-levels of x-y sections. Nuclear DNA was stained with DRAQ5 (shown in blue). (B) Every protein marker showed its specific distribution. (C) UL56 colocalized predominantly with marker proteins for TGN (TGN46, γ-adaptin and δ-adaptin), and partially with marker proteins for Golgi complex proteins (Golgi58K and GM130) and early endosomes (EEA1). Scale bars, 10 μm.