Skip to main content

Table 1 Cellular proteins identified in purified virion preparations following 1-D SDS-PAGE and LC-MS/MS

From: Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach

    

Gel pieces found in d (No. of spectra/No. unique peptides)

 

Protein name

Taxonomy a

Accession No. b

Mass (kDa)

BHK

4T1

A549

Other viruses found in e

Tubulin alpha

H

IPI00180675

50.1

3(11/9)

  

Influenza [21], HCMV [19], VV [11, 13], HIV-1 [23], ASFV [58].

 

M

IPI00110753

50.1

3(11/10)

9(2/2)

15(2/2)

 

Annexin A2

H

IPI00455315

38.6

2(8/4)

8(4/3)

14(3/2)

Influenza [21], HCMV [19, 59], VV [11], KSHV [20], HIV-1[23], HSV-1 [18], AlHV-1 [15].

 

M

IPI00468203

38.5

2(12/7)

8(5/4)

14(2/2)

 

Elongation factor 1-alpha

H

IPI00014424

50.5

3 (9/3)

9(9/4)

 

HIV-1 [23, 24, 60, 61], VV [11, 13], MCMV [17], HCMV [19], SARS-CoV [22].

 

M

IPI00307837

50.3

3(9/3)

9(13/6)

15(3/2)

 

Ubiquitin

H

IPI00719280

25.8

2(6/3),3(4/3), 4(3/2),5(3/2)

8(8/3),9(7/3), 11(2/2)

14(3/2), 17(2/2)

Influenza [21], HIV-1 [23, 24, 62], SIV [62], MMLV [25, 62], VV [11, 45], AcNPV [44], ASFV [45].

 

M

IPI00923013

26.6

2(5/2),3(4/3), 4(3/2),5(3/2)

8(7/3),9(6/2), 10(2/2), 11(2/2)

14(3/2), 15(3/2), 17(2/2)

 

Integrin beta-1

H

IPI00217561

91.7c

6(2/2)

12(3/2)

18(6/2)

Influenza [21], HIV-1 [23], MMLV [25].

 

M

IPI00132474

88.2c

6(3/3)

12(4/3)

  

Tubulin, beta

H

IPI00011654

49.7

3(11/7)

9(3/3)

 

Influenza [21], HCMV [19], EBV [16], VV [11, 13], MMLV [25], ASFV [58].

 

M

IPI00109061

49.9

3(9/6)

9(3/3)

  

Actin, cytoplasmic

H

IPI00021439

41.7

4(3/2)

8(3/3)

 

Influenza [21], HCMV [19], EBV [16], VV [11, 12, 63], KSHV [20] MMLV [25], HIV-1 [23, 39], MCMV [17], HSV-1 [18], ASFV [58], AlHV-1 [15], SeV [48], MV [49], RV [50].

 

M

IPI00110850

41.7

4(3/2)

8(3/3)

  

Elongation factor 2

M

IPI00466069

95.2

5(2/2)

11(3/2)

 

HIV-1 [23], KSHV [20], HCMV [19], SARS-CoV [22].

Transferrin receptor protein 1

H

IPI00022462

84.9c

  

17(3/2)

HSV-1 [18], VV [64].

 

M

IPI00124700

85.7c

 

11(2/2)

  

Low-density lipoprotein receptor-related protein 1, 85 kDa and 515 kDa subunits

H

IPI00020557

504.5

5(7/5), 6 (33/27)

   
 

M

IPI00119063

504.7

5(7/5), 6(38/29)

   

Hsp90

H

IPI00784295

84.8

5(8/7)

  

HIV-1[23], VV [11], EBV [16], KSHV [20], HCMV [19], SARS-CoV [22].

 

M

IPI00330804

84.6

5(8/8)

   

Neural cell adhesion molecule 1

H

IPI00435020

93.3c

2(2/2), 6(7/6)

   
 

M

IPI00122971

119.3c

6(6/5)

   

Heat shock cognate 71 kDa protein

H

IPI00003865

70.9

5 (8/6)

  

MMLV [25], HIV-1 [23, 65], VV [11], RV [10], NDV [10], Influenza [10].

 

M

IPI00323357

70.9

5(3/3)

   

Chondroitin sulfate proteoglycan 4

H

IPI00019157

250.5

6(3/3)

   
 

M

IPI00128915

252.4

6(5/5)

   

Prostaglandin F2 receptor negative regulator

H

IPI00022048

98.5c

6(4/4)

  

MMLV [25], HIV-1 [23].

 

M

IPI00125497

106.0c

6(4/4)

   

Enolase

H

IPI00216171

47.1

3(2/2)

  

Influenza [21], HCMV [19], EBV [16], KSHV [20], HIV-1 [23], VV [12].

 

M

IPI00462072

47.0

3(4/4)

   

Annexin A5

H

IPI00329801

35.8

2(5/3)

  

Influenza [21], HCMV [19], HIV-1[23], HSV-1 [18], VV [63].

 

M

IPI00317309

35.7

2(4/2)

   

Synaptic vesicle membrane protein VAT-1 homolog

H

IPI00156689

41.9

3(4/3)

   
 

M

IPI00126072

43.1

3(2/2)

   

Annexin A4

M

IPI00353727

35.8

2(3/2)

  

Influenza [21].

ATP synthase alpha chain, mitochondrial

H

IPI00440493

59.7

3(2/2)

   
 

M

IPI00130280

59.7

3(2/2)

   

ATP synthase beta chain, mitochondrial

M

IPI00468481

56.3

3(2/2)

  

HIV-1 [23], VV [63].

Casein kinase I

H

IPI00167096

39.1

2(2/2)

   
 

M

IPI00330729

38.9

2(2/2)

   

CD44 antigen

H

IPI00297160

39.4c

5(3/2)

  

HIV-1 [23].

 

M

IPI00223769

40.2c

5(2/2)

   

Fascin

M

IPI00353563

54.3

3(2/2)

  

VV [12].

Fibronectin

M

IPI00113539

272.5

12(2/2)

  

HIV-1 [23].

Guanine nucleotide-binding protein G(o), alpha subunit 2

M

IPI00115546

39.9

2(3/2)

   

Histone H3.2

M

IPI00230730

15.2

1(2/2)

   

Histone H4

H

IPI00453473

11.2

1(2/2)

  

MMLV [25], HIV-1 [23], AlHV-1 [15], SARS-CoV [22].

 

M

IPI00329998

11.4

1(2/2)

   

Methyl-CpG-binding domain protein 4

M

IPI00321709

62.6

3(2/2)

   

Monocyte differentiation antigen CD14

M

IPI00308990

39.2

3(3/2)

  

HIV-1 [23].

Pyruvate kinase

M

IPI00407130

58.0

3(2/2)

  

Influenza [21], KHSV [20], HIV-1 [23], VV [12, 13], AlHV-1 [15].

Integrin alpha-3

M

IPI00126090

116.7c

 

12(7/5)

 

HIV-1 [23].

Annexin A3

M

IPI00132722

36.2

 

8(4/4)

  

Envelope protein

M

IPI00406960

73.6

 

7(6/4)

  

Basigin

M

IPI00113869

29.7

 

9(3/3)

 

HIV-1 [23].

EH-domain-containing protein 1

H

IPI00017184

60.6

 

9(3/3)

  
 

M

IPI00126083

60.6

 

9(3/3)

  

Gag protein

M

IPI00224370

60.3

 

7(4/3)

  

Lymphocyte antigen 74

M

IPI00115558

35.0

 

8(3/3)

  

Tumor susceptibility gene 101 protein

H

IPI00018434

43.9

 

9(3/3)

 

HIV-1 [23].

 

M

IPI00117944

44.1

 

9(3/3)

  

Acid sphingomyelinase-like phosphodiesterase 3b

M

IPI00117534

51.6

 

9(2/2)

  

Dystrophin

M

IPI00474450

425.8

 

8(2/2)

  

Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit

H

IPI00003348

37.2

 

8(3/2)

  
 

M

IPI00116938

37.2

 

8(3/2)

  

H-2 class I histocompatibility antigen, D-D alpha chain

M

IPI00110805

41.1

 

9(2/2)

  

H-2 class I histocompatibility antigen, L-D alpha chain

M

IPI00109996

40.7

 

9(2/2)

  

L-lactate dehydrogenase A chain

M

IPI00319994

36.3

 

8(2/2)

 

HCMV [19], VV [12], SARS-Cov [22].

Monocarboxylate transporter 1

M

IPI00137194

53.3

 

8(2/2)

  

Myosin-9

H

IPI00019502

226.4

 

12(2/2)

 

VV [11], KHSV [20].

T-complex protein 1 subunit beta

M

IPI00320217

57.3

 

9(2/2)

 

HIV-1 [23].

Transmembrane protease, serine 11E

M

IPI00222870

50.0

 

9(2/2)

  

Ubiquitin protein ligase E3 component n-recognin 4

M

IPI00378681

572.3

 

8(2/2)

  

4F2 cell-surface antigen heavy chain

H

IPI00027493

57.9c

  

17(9/7)

HIV-1 [23].

Albumin

H

IPI00022434

71.7

  

14(3/3)

HIV-1 [23].

Annexin A1

H

IPI00218918

38.6

  

14(3/3)

Influenza [21], HCMV [19], VV [11], HIV-1[23], MCMV [17], HSV-1 [18], AlHV-1 [15].

CD109 antigen

H

IPI00152540

161.7

  

18(3/3)

 

Transmembrane protein 2

H

IPI00170706

154.4

  

18(3/3)

 

Aminopeptidase N

H

IPI00221224

109.4

  

18(2/2)

HIV-1 [23], HCMV [19].

Integrin alpha-V

M

IPI00319509

109.5

  

18(2/2)

HIV-1 [23].

 

H

IPI00027505

116.0

  

18(2/2)

 

Lutheran blood group glycoprotein

H

IPI00002406

67.4c

  

17(2/2)

 

Neutral amino acid transporter B(0)

H

IPI00019472

56.6c

  

17(2/2)

 

Programmed cell death 6 interacting protein

H

IPI00246058

96.8

  

17(2/2)

MMLV [25], HIV-1 [23].

Ras-related protein Rab-11B

M

IPI00135869

24.3

  

13(2/2)

 

Ras-related protein Rap-1A

M

IPI00138406

21.0

  

13(2/2)

HIV-1 [23].

Solute carrier family 2, facilitated glucose transporter member 1

H

IPI00220194

54.1c

  

17(2/2)

 
 

M

IPI00308691

53.9c

  

17(2/2)

 

T-complex protein 10a

M

IPI00122340

47.1

  

18 (3/2)

 
  1. a From search of human (H) or mouse (M) database
  2. b International protein index accession numbers
  3. c Glycosylated protein
  4. d Gel bands were numbered as depicted in Figure 2D
  5. e ASFV, African swine fever virus; AlHV-1, Alcelaphine herpesvirus-1; AcNPV, Autographa californica nuclear polyhedrosis virus; EBV, Epstein-Barr virus; HCMV, human cytomegalovirus; HIV-1, human immunodeficiency virus-1; KSHV, Kaposi's sarcoma-associated herpesvirus; MV, measles virus, MMLV, Moloney murine leukemia virus; MCMV, murine cytomegalovirus; NDV, Newcastle disease virus; RV, rabies virus; SeV, Sendai virus; SARS-CoV, severe acute respiratory syndrome coronavirus; SIV, simian immunodeficiency virus; VV, vaccinia virus
Back to article page

Virology Journal

ISSN: 1743-422X