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Table 1 Cellular proteins identified in purified virion preparations following 1-D SDS-PAGE and LC-MS/MS

From: Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach

     Gel pieces found in d (No. of spectra/No. unique peptides)  
Protein name Taxonomy a Accession No. b Mass (kDa) BHK 4T1 A549 Other viruses found in e
Tubulin alpha H IPI00180675 50.1 3(11/9)    Influenza [21], HCMV [19], VV [11, 13], HIV-1 [23], ASFV [58].
  M IPI00110753 50.1 3(11/10) 9(2/2) 15(2/2)  
Annexin A2 H IPI00455315 38.6 2(8/4) 8(4/3) 14(3/2) Influenza [21], HCMV [19, 59], VV [11], KSHV [20], HIV-1[23], HSV-1 [18], AlHV-1 [15].
  M IPI00468203 38.5 2(12/7) 8(5/4) 14(2/2)  
Elongation factor 1-alpha H IPI00014424 50.5 3 (9/3) 9(9/4)   HIV-1 [23, 24, 60, 61], VV [11, 13], MCMV [17], HCMV [19], SARS-CoV [22].
  M IPI00307837 50.3 3(9/3) 9(13/6) 15(3/2)  
Ubiquitin H IPI00719280 25.8 2(6/3),3(4/3), 4(3/2),5(3/2) 8(8/3),9(7/3), 11(2/2) 14(3/2), 17(2/2) Influenza [21], HIV-1 [23, 24, 62], SIV [62], MMLV [25, 62], VV [11, 45], AcNPV [44], ASFV [45].
  M IPI00923013 26.6 2(5/2),3(4/3), 4(3/2),5(3/2) 8(7/3),9(6/2), 10(2/2), 11(2/2) 14(3/2), 15(3/2), 17(2/2)  
Integrin beta-1 H IPI00217561 91.7c 6(2/2) 12(3/2) 18(6/2) Influenza [21], HIV-1 [23], MMLV [25].
  M IPI00132474 88.2c 6(3/3) 12(4/3)   
Tubulin, beta H IPI00011654 49.7 3(11/7) 9(3/3)   Influenza [21], HCMV [19], EBV [16], VV [11, 13], MMLV [25], ASFV [58].
  M IPI00109061 49.9 3(9/6) 9(3/3)   
Actin, cytoplasmic H IPI00021439 41.7 4(3/2) 8(3/3)   Influenza [21], HCMV [19], EBV [16], VV [11, 12, 63], KSHV [20] MMLV [25], HIV-1 [23, 39], MCMV [17], HSV-1 [18], ASFV [58], AlHV-1 [15], SeV [48], MV [49], RV [50].
  M IPI00110850 41.7 4(3/2) 8(3/3)   
Elongation factor 2 M IPI00466069 95.2 5(2/2) 11(3/2)   HIV-1 [23], KSHV [20], HCMV [19], SARS-CoV [22].
Transferrin receptor protein 1 H IPI00022462 84.9c    17(3/2) HSV-1 [18], VV [64].
  M IPI00124700 85.7c   11(2/2)   
Low-density lipoprotein receptor-related protein 1, 85 kDa and 515 kDa subunits H IPI00020557 504.5 5(7/5), 6 (33/27)    
  M IPI00119063 504.7 5(7/5), 6(38/29)    
Hsp90 H IPI00784295 84.8 5(8/7)    HIV-1[23], VV [11], EBV [16], KSHV [20], HCMV [19], SARS-CoV [22].
  M IPI00330804 84.6 5(8/8)    
Neural cell adhesion molecule 1 H IPI00435020 93.3c 2(2/2), 6(7/6)    
  M IPI00122971 119.3c 6(6/5)    
Heat shock cognate 71 kDa protein H IPI00003865 70.9 5 (8/6)    MMLV [25], HIV-1 [23, 65], VV [11], RV [10], NDV [10], Influenza [10].
  M IPI00323357 70.9 5(3/3)    
Chondroitin sulfate proteoglycan 4 H IPI00019157 250.5 6(3/3)    
  M IPI00128915 252.4 6(5/5)    
Prostaglandin F2 receptor negative regulator H IPI00022048 98.5c 6(4/4)    MMLV [25], HIV-1 [23].
  M IPI00125497 106.0c 6(4/4)    
Enolase H IPI00216171 47.1 3(2/2)    Influenza [21], HCMV [19], EBV [16], KSHV [20], HIV-1 [23], VV [12].
  M IPI00462072 47.0 3(4/4)    
Annexin A5 H IPI00329801 35.8 2(5/3)    Influenza [21], HCMV [19], HIV-1[23], HSV-1 [18], VV [63].
  M IPI00317309 35.7 2(4/2)    
Synaptic vesicle membrane protein VAT-1 homolog H IPI00156689 41.9 3(4/3)    
  M IPI00126072 43.1 3(2/2)    
Annexin A4 M IPI00353727 35.8 2(3/2)    Influenza [21].
ATP synthase alpha chain, mitochondrial H IPI00440493 59.7 3(2/2)    
  M IPI00130280 59.7 3(2/2)    
ATP synthase beta chain, mitochondrial M IPI00468481 56.3 3(2/2)    HIV-1 [23], VV [63].
Casein kinase I H IPI00167096 39.1 2(2/2)    
  M IPI00330729 38.9 2(2/2)    
CD44 antigen H IPI00297160 39.4c 5(3/2)    HIV-1 [23].
  M IPI00223769 40.2c 5(2/2)    
Fascin M IPI00353563 54.3 3(2/2)    VV [12].
Fibronectin M IPI00113539 272.5 12(2/2)    HIV-1 [23].
Guanine nucleotide-binding protein G(o), alpha subunit 2 M IPI00115546 39.9 2(3/2)    
Histone H3.2 M IPI00230730 15.2 1(2/2)    
Histone H4 H IPI00453473 11.2 1(2/2)    MMLV [25], HIV-1 [23], AlHV-1 [15], SARS-CoV [22].
  M IPI00329998 11.4 1(2/2)    
Methyl-CpG-binding domain protein 4 M IPI00321709 62.6 3(2/2)    
Monocyte differentiation antigen CD14 M IPI00308990 39.2 3(3/2)    HIV-1 [23].
Pyruvate kinase M IPI00407130 58.0 3(2/2)    Influenza [21], KHSV [20], HIV-1 [23], VV [12, 13], AlHV-1 [15].
Integrin alpha-3 M IPI00126090 116.7c   12(7/5)   HIV-1 [23].
Annexin A3 M IPI00132722 36.2   8(4/4)   
Envelope protein M IPI00406960 73.6   7(6/4)   
Basigin M IPI00113869 29.7   9(3/3)   HIV-1 [23].
EH-domain-containing protein 1 H IPI00017184 60.6   9(3/3)   
  M IPI00126083 60.6   9(3/3)   
Gag protein M IPI00224370 60.3   7(4/3)   
Lymphocyte antigen 74 M IPI00115558 35.0   8(3/3)   
Tumor susceptibility gene 101 protein H IPI00018434 43.9   9(3/3)   HIV-1 [23].
  M IPI00117944 44.1   9(3/3)   
Acid sphingomyelinase-like phosphodiesterase 3b M IPI00117534 51.6   9(2/2)   
Dystrophin M IPI00474450 425.8   8(2/2)   
Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit H IPI00003348 37.2   8(3/2)   
  M IPI00116938 37.2   8(3/2)   
H-2 class I histocompatibility antigen, D-D alpha chain M IPI00110805 41.1   9(2/2)   
H-2 class I histocompatibility antigen, L-D alpha chain M IPI00109996 40.7   9(2/2)   
L-lactate dehydrogenase A chain M IPI00319994 36.3   8(2/2)   HCMV [19], VV [12], SARS-Cov [22].
Monocarboxylate transporter 1 M IPI00137194 53.3   8(2/2)   
Myosin-9 H IPI00019502 226.4   12(2/2)   VV [11], KHSV [20].
T-complex protein 1 subunit beta M IPI00320217 57.3   9(2/2)   HIV-1 [23].
Transmembrane protease, serine 11E M IPI00222870 50.0   9(2/2)   
Ubiquitin protein ligase E3 component n-recognin 4 M IPI00378681 572.3   8(2/2)   
4F2 cell-surface antigen heavy chain H IPI00027493 57.9c    17(9/7) HIV-1 [23].
Albumin H IPI00022434 71.7    14(3/3) HIV-1 [23].
Annexin A1 H IPI00218918 38.6    14(3/3) Influenza [21], HCMV [19], VV [11], HIV-1[23], MCMV [17], HSV-1 [18], AlHV-1 [15].
CD109 antigen H IPI00152540 161.7    18(3/3)  
Transmembrane protein 2 H IPI00170706 154.4    18(3/3)  
Aminopeptidase N H IPI00221224 109.4    18(2/2) HIV-1 [23], HCMV [19].
Integrin alpha-V M IPI00319509 109.5    18(2/2) HIV-1 [23].
  H IPI00027505 116.0    18(2/2)  
Lutheran blood group glycoprotein H IPI00002406 67.4c    17(2/2)  
Neutral amino acid transporter B(0) H IPI00019472 56.6c    17(2/2)  
Programmed cell death 6 interacting protein H IPI00246058 96.8    17(2/2) MMLV [25], HIV-1 [23].
Ras-related protein Rab-11B M IPI00135869 24.3    13(2/2)  
Ras-related protein Rap-1A M IPI00138406 21.0    13(2/2) HIV-1 [23].
Solute carrier family 2, facilitated glucose transporter member 1 H IPI00220194 54.1c    17(2/2)  
  M IPI00308691 53.9c    17(2/2)  
T-complex protein 10a M IPI00122340 47.1    18 (3/2)  
  1. a From search of human (H) or mouse (M) database
  2. b International protein index accession numbers
  3. c Glycosylated protein
  4. d Gel bands were numbered as depicted in Figure 2D
  5. e ASFV, African swine fever virus; AlHV-1, Alcelaphine herpesvirus-1; AcNPV, Autographa californica nuclear polyhedrosis virus; EBV, Epstein-Barr virus; HCMV, human cytomegalovirus; HIV-1, human immunodeficiency virus-1; KSHV, Kaposi's sarcoma-associated herpesvirus; MV, measles virus, MMLV, Moloney murine leukemia virus; MCMV, murine cytomegalovirus; NDV, Newcastle disease virus; RV, rabies virus; SeV, Sendai virus; SARS-CoV, severe acute respiratory syndrome coronavirus; SIV, simian immunodeficiency virus; VV, vaccinia virus