Figure 4

Loss of S2 dsRNA by treatment with anisomycin. Cultures of strain UCD 67-385 were made in YM broth supplemented with different concentrations of anisomycin (A). Culture samples were removed at 42 and 50 h and streaked for single colonies onto YM medium. The total RNA was extracted from cultures of each colony and the content of dsRNA elements was determined (B). The dsRNA pattern of two strains cured of S2 dsRNA [385(S2)-40 and 385(S2)-42] and uncured (UCD 67-385) strains are shown.