Figure 5

Analysis of viral protein and DNA in HAV5.EGFP capsids. (A) Proteins from lysates of RNase/DNase treated or untreated mature and empty/intermediate capsids were separated by 10% SDS-PAGE, and stained by SYPRO Ruby protein stains. A 100 kDa hexon protein band was used for determining the capsid protein concentration using Kodak IM Network software. (B) Total yields of DNAs isolated from mature and empty/intermediate capsids with or without RNase/DNase treatment. (C) EGFP expression. 293 cells were transduced by 10 fold serially diluted mature and empty/intermediate capsids with or without RNase/DNase treatment. At 48 h post transduction, cells were analysed for EGFP expression by a fluorescent microscope (i) EGFP, (ii) Phase contrast. Presence (+). Absence (-). Mature (Ma); Empty/Intermediate (E-I).