Figure 3

Quantitation of viral RNAs by real-time PCR. Viral genomic DNA and RNAs were isolated from the same pool of purified mature capsids. RNAs with (+) or without (-) RNase treatment were reversely transcribed with oligo-dT and hexamers, and then used as templates in the real-time PCR with TaqMan probes. The calculated copy number of the mRNAs of E1B_small and fiber is shown as percentage relative to the copy number of the viral genomic DNA. The averaged cycle threshold (Ct) values of 5 replicates were used to determine the relative RNA copy number.