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Table 3 Kd Values for Wild-Type Reverse Transcriptases and Their Chimeric Derivatives

From: Influence of the RNase H domain of retroviral reverse transcriptases on the metal specificity and substrate selection of their polymerase domains

Enzymes

Kd (DNA)

(nM)

Wild-type HIV-1 RT

3.20

Chimeric HIV-1 RT

1.50

Wild-type MuLV RT

2.70

Chimeric MuLV RT

3.3

M-pol

1.2

  1. The dissociation constant was determined by a mobility shift assay using a hetero-polymeric 49/17-mer template primer. The values represent the average of three independent experiments.
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Virology Journal

ISSN: 1743-422X