Figure 3
Long-term inhibition of HCV RNA by EBNA1/OriP and wild-type baculovirus-mediated sh452. A Real-time RT-PCR analysis of HCV RNA expression after transduction of HCV full replicon cells (NNC#2, 4 × 104 cells/well) with Ac-shRNA452 (MOI = 100 [circle]), Ac-EP-shRNA452 (MOI = 100 [square]). B The cytotoxicity of Ac-EP-shRNA452 (square) and Ac-shRNA452 (circle) represented as the percentage reduction of viable Huh-7 cells. A cytotoxicity assay was performed using a BrdU Cell Proliferation ELISA kit according to the manufacturer's instructions (Roche Diagnostics GmbH). The toxicity results are representative of three independent experiments.