Figure 2

Inhibition of HCV RNA by EBNA1/OriP and wild-type baculovirus-mediated sh452. A Real time PCR analysis of HCV RNA expression after transduction of HCV full replicon cells (NNC#2, 4 × 10⁴ cells/well) with an MOI 50 and 100 baculovirus-mediated shRNA. HCV RNA values relative to the scrambled shRNA control are shown. B Inhibition of HCV replication by baculovirus-mediated core shRNAs. Ac-shRNAs were used to infect HCV replicons and intracellular HCV core protein levels measured after 3 days by an HCV protein antigen CLEIA assay. Error bars represent standard errors of the mean from three experiments. *p < 0.01.