Comparison of replication efficiencies of both wild type (VA) and various mutants [VA( AC2M ), VA( AC4M ) and VA( AC2MAC4M )] of VA/ToLCV constructs in tomato plants. (A) Total DNA was isolated from the tomato leaves agroinfiltrated with VIGS, VA(AC2M), VA(AC4M) and VA(AC2MAC4M) separately and the release of episome at indicated dpi was checked by PCR using internal primers. The respective ACTIN controls are shown. (B) The band intensities of the amplified products were quantified and the normalized values with respect to corresponding loading controls (ACTIN) are plotted as bar graphs. The corresponding value for VA construct at 3 dpi was arbitrarily assigned as 100%. The standard deviations based on three independent experiments are shown.