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Figure 3 | Virology Journal

Figure 3

From: A geminiviral amplicon (VA) derived from Tomato leaf curl virus (ToLCV) can replicate in a wide variety of plant species and also acts as a VIGS vector

Figure 3

Northern hybridization showing the time kinetics of siRNAs accumulation in tomato plants agroinfiltrated with VA/pCAMBIA1391Z. Northern hybridization using CR-AC3 DNA fragment as a probe showing the formation of siRNAs in infiltrated tomato leaves at indicated time points (dpi). A labeled 21-mer oligonucleotide was used as a size marker. (B) The relative intensities of the siRNA bands were estimated and the normalized values with respect to corresponding loading controls are plotted as bar graphs. The value at 7 dpi was arbitrarily assigned as 100%. The standard deviations based on three independent experiments are shown.

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