Table 1 Detection of IBV in CAM and chicken embryo.
Sample No. | Time after inocul ation (h PI) | Embryo status | Results of Immunohistochemistry | Virological findings‡ (TCID50/ml) |
|---|---|---|---|---|
1 | 24 | Dead | CAM - | 0 |
2 | 24 | Dead | CAM + | 104.5 |
3 | 24 | Dead | CAM + | 104.5 |
4 | 41 | Dead | CAM + | 104 |
5 | 41 | Dead | CAM + | 103 |
Gizzard (smooth muscle, vasculature) ++ | ||||
Heart (vasculature) ++ | ||||
Kidney (glomerular tufts) ++ | ||||
Liver (Kupffer cells) ++ | ||||
Lung (vasculature) ++ | ||||
Proventriculus (smooth muscle) ++ | ||||
Spleen (macrophages) + | ||||
6 | 48 | Alive | CAM + | 104 |
Gizzard ++ | ||||
Heart (vasculature) ++ | ||||
Kidney (glomerular tufts) ++ | ||||
Liver (Kupffer cells) + | ||||
Lung ++ | ||||
Proventriculus + | ||||
Spleen (Macrophages) +++ | ||||
7 | 48 | Alive | CAM + | 106 |
Nasal mucosa (epithelium) ++ | ||||
Skin (epidermis) + | ||||
8 | 48 | Alive | CAM + | 105 |
Nasal mucosa (epithelium) ++ | ||||
Skin (epidermis) + | ||||
9 | 65 | Dead | Brain (neurons) + | 105 |
CAM: + | ||||
Eye (sclera) + | ||||
Gizzard ++ | ||||
Liver + | ||||
Spinal cord (neurons) + | ||||
Spleen (Macrophages) ++ | ||||
10 | 72 | Alive | CAM: + | 106 |
Gizzard (mucosa) ++ | ||||
Heart (vasculature) | ||||
Kidney (tubuli) + | ||||
Nasal cavity (mucosa) +++ | ||||
Skin (epidermis) ++ | ||||
Spinal cord (neurons) + | ||||
Spleen (macrophages) ++ | ||||
11 | 87 | Dead | CAM + | 105 |
Kidney (glomeruli) + | ||||
Liver (Kupffer cells) + | ||||
Lungs (epithelium, vasculature) + | ||||
Nasal cavity (mucosa)++ | ||||
Proventriculus (smooth muscle, mucosa) ++ | ||||
Skin (epidermis) ++ | ||||
Spleen (macrophages) +++ | ||||
12 | 120 | Dead | Brain (neurons) + | 102 |
CAM + | ||||
Nasal cavity (mucosa) ++ | ||||
Proventriculus (mucosa) + | ||||
Skin (epidermis) ++ | ||||
Trachea (mucosa) ++ |