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Figure 1 | Virology Journal

Figure 1

From: Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo

Figure 1

Establishment of the fluorescent quantitative real-time PCR (FQ-PCR) standard curve. Ten-fold dilutions of standard DNA ranging from 2.8 × 108 to 2.8 × 104 copies/μL were used, as indicated on the x-axis, whereas the corresponding cycle threshold (Ct) values are presented on the y-axis. Each dot represents the result of triplicate amplifications of each dilution. The correlation coefficient and slope value of the regression curve were calculated and are indicated. (1:2.8 × 108, Ct = 12.7; 2: 2.8 × 107, Ct = 16.2; 3: 2.8 × 106, Ct = 19.4; 4: 2.8 × 105, Ct = 22.9; 5: 2.8 × 104, Ct = 25.9)

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Virology Journal

ISSN: 1743-422X