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Figure 6 | Virology Journal

Figure 6

From: Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo

Figure 6

Expression of ORF 7b. (A) Detection of ORF 7b-flag expression with an anti-flag antibody by Western blot analysis. The 10 kD band is non-specifically detected in all samples. (B) Vero cells were infected with the flag-tagged recombinant virus rSCV7bflag or with the recombinant virus rSCV and subjected to IFA at 24 h p.i. IFA was done with anti-flag antibody (left panel, anti-flag) or a convalescent patient serum reacting predominantly against the SARS-CoV nucleocapsid protein (right panel, anti N).

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Virology Journal

ISSN: 1743-422X