Figure 4

Inhalation of attenuated mengovirus enhanced pulmonary expression of the chemokines, CINC-1, MIP-2, and MCP-1. BAL fluid was harvested on days 1, 3, and 5 postinoculation from the right lungs of BN rats inoculated with 10⁷ PFU of vMC₀ (n = 4, 10, and 4 rats, respectively) and on day 3 postinoculation from those inoculated with an equivalent amount of UV-inactivated vMC₀ (n = 5 rats) or vehicle (n = 5–6 rats), and (A) CINC-1, (B) MIP-2, and (C) MCP-1 levels were determined by ELISA. Data are the total amount of chemokine recovered from the right lung BAL (ELISA values, corrected for the 15× concentration, were multiplied by the BAL fluid volume). (A, B) Data are presented as box plots. (C) Symbols represent data from individual rats; bars indicate medians. * P < 0.05, ‡ P = 0.05 (vMC₀ vs. vehicle); † P < 0.05 (vMC₀ vs. UV-inactivated vMC₀).