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Figure 1 | Virology Journal

Figure 1

From: In vitro dynamics of HIV-1 BF intersubtype recombinants genomic regions involved in the regulation of gene expression

Figure 1

Detection of BF intersubtype recombinant genomes by PCR amplification. Proviral DNA found in samples from mono (B or F Subtype) and dual-infected (B+F) cultures was used to obtain four different amplicons from the genomics regions under study. Primers combinations and viral strains (B, F or FB) present in each cell culture are indicated (as described in Methods). Upper and lower panels show the result of the amplification of the LTR-Gag and Vpr/Tat regions, respectively. Intersubtype recombinant PCR products are indicated by arrows. Positive and negative controls were included in the reactions.

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Virology Journal

ISSN: 1743-422X