Figure 1
From: Optimization of capsid-incorporated antigens for a novel adenovirus vaccine approach
Construction of hexon modified genomes. (A) Agarose gel electrophoresis of PCR products were obtained through PCR of genomic DNA of Ad5 using epitope specific primers (Table 1). (B) HVR2 or HVR5 shuttle vector sites which were modified with a RGD motif. The DNA encoding for the respective RGD motifs were cloned into the HVRs of our previously modified shuttle vectors within the LGSHHHHHLGS linker, as indicated by the arrows.