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Table 1 Comparison of the detection of human and bovine noroviruses by the conventional RT-PCR assay and the SYBR Green assay

From: A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition

   SYBR Green RT-PCR  
   + - Inhibition  
Conventional + 44 50 0 0 6 0 50
RT-PCR - 1 4 13 32 22 0 36
   45 54 13 32 28 0 86
  1. Normal font: SYBR Green assay on RNA directly; bold font: SYBR Green assay modified to remove inhibition using 10-fold dilution of extracted RNA.