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Table 1 Comparison of the detection of human and bovine noroviruses by the conventional RT-PCR assay and the SYBR Green assay

From: A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition

  

SYBR Green RT-PCR

 
  

+

-

Inhibition

 

Conventional

+

44

50

0

0

6

0

50

RT-PCR

-

1

4

13

32

22

0

36

  

45

54

13

32

28

0

86

  1. Normal font: SYBR Green assay on RNA directly; bold font: SYBR Green assay modified to remove inhibition using 10-fold dilution of extracted RNA.