Figure 2

Detection of RVFV Gn and Gc and the cell surface expression. A) Cell lysate from infected cells with a recombinant baculovirus expressing RVFV N, Gn and Gc were analyzed by SDS-PAGE followed by Commassie blue stain (lane2). As a control cell lysate from uninfected cells were included (lane 1). B) Western blot using specific antibodies against Gn (lane 2) or Gc (lane 5) was performed with cell lysate expressing RVFV proteins N, Gn and Gc. As a control cell lysates from uninfected cells (lanes 1 and 4) or expressing RVFV N protein (lanes 3 and 6) were included. C) Cell surface expression of RVFV Gn. Infected cells expressing RVFV N, Gn and Gc proteins were fixed and processed for immunoflorescence under non-permeabilizing conditions. To detect RVFV Gn protein, a specific antibody was used followed by an anti-mouse-FITC conjugated secondary antibody (upper panel). As control cells expressing β-Gal protein were processed similarly (lower panel). D) Cell surface expression of RVFV Gc. Cells expressing RVFV N, Gn and Gc were examined for cell surface expression of Gc using a specific antibody against Gc and a anti mouse-TRITC as secondary antibody (upper panel). Control cells were included (lower panel).