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Table 2 Identification of all neuraminidase subtypes by RT-PCR followed by direct sequencing using animal samples from allantoic fluids.

From: A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus

Influenza virus strain (a)

Subtype (b)

One-step RT-

PCR (c)

NA subtype by

sequence (d,e)

1) A/Chicken/Vietnam/8/04

H5N1

+

N1

2) A/Chicken/Laos/26/06

H5N1

+ (8)

N1

3) A/Chicken/Cambodia/1A/04

H5N1

+

N1

4) A/Chicken/Malacca/4905/03

H9N2

+ (1)

N2

5) A/Gull/Denmark/68110/02

H16N3

+ (2)

N3

6) A/Shearwater/Aust/75

H5N3

+

N3

7) A/Grey teal/WA/1762/79

H4N4

+

N4

8) A/Emu/NSW/97

H7N4

+

N4

9) A/Turkey/Ontario/6118/67

H8N4

+ (3)

N4

10) A/Shearwater/Aust/72

H6N5

+ (10)

N5

11) A/Mallard/Gurjev/263/82

H14N5

+ (4)

N5

12) A/Mallard/Gurjev/244/82

H14N6

+ (12)

N6

13) A/Gull/Maryland/704/77

H13N6

Neg (5, 11)

Not typed

14) A/Gull/Tas/06

H13N6

+

N6

15) A/Duck/NZ/89

H4N6

+

N6

16) A/Grey teal/WA/1855

H4N6

+

N6

17) A/Duck/Viet/317/2005

H4N6

+

N6

18) A/Duck/Viet/318/2005

H4N6

+

N6

19) A/Duck/Viet/323/2005

H4N6

+

N6

20) A/Duck/Viet/342/2005

H3N6

+

N6

21) A/Duck/Vic/512/2007

H7N6

+

N6

22) A/Duck/Victoria/1/76

H7N7

+ (13)

N7

23) A/Chicken/Germany/N/49

H10N7

+ (6)

N7

24) A/Chicken/Victoria/1/85(f)

H7N7

+

N7

25) A/N. Korean

H7N7

+

N7

26) A/Avian/669/WA/78

H3N8

+ (14)

N8

27) A/Equine/Sydney/2888-8/2007

H3/N8

+

N8

28) A/Tern/Aust/75

H11N9

+ (7, 15)

N9

29) A/Shelduck/WA/1757/78(f)

H1N9

+

N9

30) A/Red-necked stint/WA/5745/84(f)

H12N9

+

N9

31) A/Shelduck/WA/1762/79

H15N9

+

N9

32) A/Wedge tailed shearwater/WA/2327/1983

H15N9

+

N9

  1. (a) Strains from the collection at the Australian Animal Health Laboratory were kindly provided by Paul Selleck. RNA was extracted from allantoic fluid.
  2. (b) Subtypes of virus stock were determined previously at AAHL by HA and NA inhibition assays according to Barr and O'Rourke [38], Van Dusen, et al. [18]and Aymard-Henry, et al. [17].
  3. (c) RT-PCR fragment visualized in agarose gel electrophoresis. In brackets refer to lane numbers on agarose gel, Figure 3; not all results shown on gel)
  4. (d) Sequencing directly from gel purified one-step RT-PCR using M13 tagged primers.
  5. (e) Results of Blastn analysis.
  6. (f) These samples were assayed by two-step RT-PCR before transferring to one-step RT-PCR.