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Table 2 Identification of all neuraminidase subtypes by RT-PCR followed by direct sequencing using animal samples from allantoic fluids.

From: A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus

Influenza virus strain (a) Subtype (b) One-step RT-
PCR (c)
NA subtype by
sequence (d,e)
1) A/Chicken/Vietnam/8/04 H5N1 + N1
2) A/Chicken/Laos/26/06 H5N1 + (8) N1
3) A/Chicken/Cambodia/1A/04 H5N1 + N1
4) A/Chicken/Malacca/4905/03 H9N2 + (1) N2
5) A/Gull/Denmark/68110/02 H16N3 + (2) N3
6) A/Shearwater/Aust/75 H5N3 + N3
7) A/Grey teal/WA/1762/79 H4N4 + N4
8) A/Emu/NSW/97 H7N4 + N4
9) A/Turkey/Ontario/6118/67 H8N4 + (3) N4
10) A/Shearwater/Aust/72 H6N5 + (10) N5
11) A/Mallard/Gurjev/263/82 H14N5 + (4) N5
12) A/Mallard/Gurjev/244/82 H14N6 + (12) N6
13) A/Gull/Maryland/704/77 H13N6 Neg (5, 11) Not typed
14) A/Gull/Tas/06 H13N6 + N6
15) A/Duck/NZ/89 H4N6 + N6
16) A/Grey teal/WA/1855 H4N6 + N6
17) A/Duck/Viet/317/2005 H4N6 + N6
18) A/Duck/Viet/318/2005 H4N6 + N6
19) A/Duck/Viet/323/2005 H4N6 + N6
20) A/Duck/Viet/342/2005 H3N6 + N6
21) A/Duck/Vic/512/2007 H7N6 + N6
22) A/Duck/Victoria/1/76 H7N7 + (13) N7
23) A/Chicken/Germany/N/49 H10N7 + (6) N7
24) A/Chicken/Victoria/1/85(f) H7N7 + N7
25) A/N. Korean H7N7 + N7
26) A/Avian/669/WA/78 H3N8 + (14) N8
27) A/Equine/Sydney/2888-8/2007 H3/N8 + N8
28) A/Tern/Aust/75 H11N9 + (7, 15) N9
29) A/Shelduck/WA/1757/78(f) H1N9 + N9
30) A/Red-necked stint/WA/5745/84(f) H12N9 + N9
31) A/Shelduck/WA/1762/79 H15N9 + N9
32) A/Wedge tailed shearwater/WA/2327/1983 H15N9 + N9
  1. (a) Strains from the collection at the Australian Animal Health Laboratory were kindly provided by Paul Selleck. RNA was extracted from allantoic fluid.
  2. (b) Subtypes of virus stock were determined previously at AAHL by HA and NA inhibition assays according to Barr and O'Rourke [38], Van Dusen, et al. [18]and Aymard-Henry, et al. [17].
  3. (c) RT-PCR fragment visualized in agarose gel electrophoresis. In brackets refer to lane numbers on agarose gel, Figure 3; not all results shown on gel)
  4. (d) Sequencing directly from gel purified one-step RT-PCR using M13 tagged primers.
  5. (e) Results of Blastn analysis.
  6. (f) These samples were assayed by two-step RT-PCR before transferring to one-step RT-PCR.