Figure 5

Sensitivity of the one-step RT-PCR assay. Example of amplification results of ten-fold serial dilutions of in-vitro transcribed RNA H10N7 subtype (refer to Table 2 for strain name). A band of approximately 253 bp was clearly visible with 40 femtogram of starting RNA (equivalent to 10⁵ copies). M, 100 bp DNA Hyperladder II (Bioline); and negative control (- ctrl: water instead of template).