Table 1 Oligonucleotides used in this study (restriction sites are in bold)
From: A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
Oligonucleotides used for: | 5'-3' sequences | length of amplified fragments (bp) |
|---|---|---|
Amplification of E3 flanking regions | CGCGACGCGT TTCGACAGGGCTAC | |
CGCGACGCGT GTTTCAGGCGCAGTTG | 2731 | |
CCCTAGATCTAGA AATGGACG | ||
GCGTCTAGA TCCAATATTCTGGGTCC | 2013 | |
Insertion of TCS in adenovirus genome | GATAACAGATTTAAAT CCTTCGAA CAGAATCGAT | |
GGCCATCGAT TCTGTTCGAA GGATTTAAAT CTGTT | ||
PCR to check pAd5CMV/TCS | CGTGTCATATGGATACACGGG | |
TCCAGCATGGCTACAACCTC | 2643 | |
EGFP amplification from pEGFPC3 | AGGAAAAAAATTTAAAT CCACCATGGTGAGCAAGGGCGAGGAGCT | |
AGGAAAAAAATCGAT CGCGTTAAGATACATTGAGTTTGGAC | 1034 | |
PCR to check pAd5CMV-EGFP | GGCACCAAAATCAACGGGAC | |
AGGAAAAAAATCGATCGCGTTAAGATTACATTGAGTTTGGAC | 2312 | |
Amplification of TK from pMBP-TK | AGGAAAAAAATTTAAAT GCGCGTATGGCTTCGTAC | |
AGGAAAAAAATTTAAAT GAGTTAGCCTCCCCCATC | 1129 | |
AGGAAAAAATTCGAA TCAGTTAGCCTCCCCCATC | 1129 |