|
Immunizing Virusa
|
Mean virus titer post immunizationb (log10 PFU/gm tissue +/- SE)
|
Mean virus titer post challengec (log10 PFU/gm tissue +/- SE)
|
|---|
| |
NT
|
Lungs
|
NT
|
Lungs
|
|---|
|
wt hMPV/NL/1/00
|
5.9 +/- 0.3
|
4.6 +/- 1.4
|
<0.4 +/- 0.1
|
<0.4 +/- 0.1
|
|
rhMPV
|
6.0 +/- 0.6
|
5.1 +/- 0.5
|
<0.4 +/- 0.1
|
<0.4 +/- 0.1
|
|
rhMPV/ΔM2-2
|
2.3 +/- 0.6
|
3.3 +/- 0.4
|
<0.4 +/- 0.1
|
<0.4 +/- 0.1
|
|
placebo
|
ND
|
ND
|
5.6 +/- 0.6
|
4.5 +/- 1.5
|
- a Syrian golden hamsters, in groups of 8, were infected intranasally with 106 PFU/animal of the immunizing virus or placebo.
- b 4 animals per group were sacrificed on day 4 p.i.. Nasal turbinates and lungs were harvested and virus titers were determined by plaque assay.
- c 28 days posit immunization, 4 animals per group were challenged with 106 PFU/animal of wt hMPV/NL/1/00. 4 days post challenge, the animals were sacrificed. Nasal turbinates and lungs were harvested and virus titers were determined by plaque assay.
