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Table 1 Frequency of GFP fluorescence in Vero cells infected with rhMPVs containing GFPpolyA insert.

From: Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters

Inoculum (PFU/per well):

1

10

100

1000

MOI (PFU/cell):

0.0001

0.001

0.01

0.1

 

Positive*/total wells

Positive*/total wells

Positive*/total wells

Positive*/total wells

rhMPV/GFPpolyA, P2

ND

ND

0/96

0/288

rhMPV/ΔM2-2/GFPpolyA, P2

0/96

25/384

124/192

384/384

  1. * A well was scored as positive if GFP fluorescence was observed 4 days p.i.
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Virology Journal

ISSN: 1743-422X