Figure 4
From: In vitro effects of selenium deficiency on West Nile virus replication and cytopathogenicity
Se deficiency increases cellular oxidative status. Vero cells grown in control, Se- and Se+ media in 96-well plates were incubated with 5 μM of 2',7' dichlorodihydrofluorescein diacetate (H2DCF-DA) for 30 min, washed twice with PBS and the cell fluorescence, an indicator of overall OS, was read at 485 Ex/535 Em. Arbitrary fluorescence units for each sample, representative of mean ± SD of two independent experiments performed in triplicate are given. *p < 0.05 and **p < 0.05 as compared to control and Se- cells, respectively.