Figure 2

Effects of Se deficiency on GPx1 enzyme. In vitro Se deficiency was tested by loss of GPx1 enzyme activity. Total soluble proteins were extracted from control, Se- and Se+ Vero (A) and SK-N-SH (B) cells and GPx1 enzyme activity was measured at days 3, 7 and 10 post-induction of Se deficiency by using the cGPx1 assay kit. Data are reported as mean ± SD of triplicate experiments. * p < 0.05, ** p < 0.005 compared to control cells. (C) Analyses of GPx1 protein by Western blot. 50 μg of total protein extracted from Vero and SK-N-SH cells grown in control, Se- and Se+ media were separated on PAGE, followed by immunoblotting with anti-GPx1. Equal loading was confirmed by re-blotting the same membranes with anti-β-actin.