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Table 1 List of Methylisatin-β-thiosemicarbazone (MIBT) derivatives and the CC50 on PS and BHK-21 cells

From: N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo

Compounds *CC50 on PS cell line *CC50 on BHK-21 cell line
1 SF3 47 μg/ml 86 ug/ml
2 SF7 43 μg/ml 200 ug/ml
3 SCH16 76 μg/ml 126 ug/ml
4 SF17 21 μg/ml 42 ug/ml
5 SCH17 41 μg/ml 46 ug/ml
6 SC18 17 μg/ml 82 ug/ml
7 SCH19 18 μg/ml 141 ug/ml
8 SC18 31.5 μg/ml 140 ug/ml
9 SB18 22.5 ug/ml 36 ug/ml
10 SF24 25.5 ug/ml 16.8 ug/ml
11 SF27 22.5 ug/ml 51 ug/ml
12 SC27 21 ug/ml 46 ug/ml
13 SC28 21 ug/ml 94 ug/ml
14 SB29 25 ug/ml 21.5 ug/ml
15.Ribavirin 50 ug/ml 200 ug/ml
  1. *CC50 = The concentration of the compound that reduced the viability of cells to 50% of the control. Note: The 14 MIBT compounds belonged to four different categories based on the halogen or methyl group substituted at the position R and are designated as SB group with bromine, SC group with chlorine, SF group with fluorine and SCH group with -CH3 group substituted at R. R' has N-substituted aromatic side chain attached to the -CH2 moiety. Cytotoxicity concentration (CC50) of synthesized compounds was evaluated on exponentially growing PS and BHK-21 cells. It can be observed that the CC50 of MIBT derivatives ranged from ≥ 76 ug/ml to ≥ 17 ug/ml, while CC50 on BHK-21 cell line ranged from ≥ 200 ug/ml to ≥ 16.8 ug/ml.