Figure 4From: N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivoWestern blot illustrating the effect of SCH 16 on JEV translation using an in vitro translation kit. Lane 1-uninfected cell control, lanes 2 and 4 – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at 4 and 10 hours post infection respectively. Lanes 3 and 5 – in vitro translation product of RNA obtained from JEV infected PS cells treated with SCH 16 for 4 hours and 10 hours respectively. Note that SCH 16 treatment of JEV infected cells did not show any in vitro translation product at 4 hours post treatment (Lane 3) whilst at 10 hours (Lane 5) a 50 Kda in vitro translation product was obtained. Lane M represents molecular weight markers.Back to article page