Western blot illustrating the effect of SCH 16 on JEV translation using an in vitro translation kit. Lane 1-uninfected cell control, lanes 2 and 4 – in vitro translation products of RNA obtained form JEV infected PS cells (untreated) at 4 and 10 hours post infection respectively. Lanes 3 and 5 – in vitro translation product of RNA obtained from JEV infected PS cells treated with SCH 16 for 4 hours and 10 hours respectively. Note that SCH 16 treatment of JEV infected cells did not show any in vitro translation product at 4 hours post treatment (Lane 3) whilst at 10 hours (Lane 5) a 50 Kda in vitro translation product was obtained. Lane M represents molecular weight markers.