Figure 5

CXCR4 expression is reduced in Jurkat cells after induction of HIV-1 Env expression. After 4 days induction of HIV-1 Env proteins, non-induced and induced cells were fixed and labeled with a mouse MAb to CXCR4 (12G5) and a secondary FITC-conjugated anti-mouse antibodies for observation with a fluorescence microscopy. Panels A and C: CXCR4 staining of non-induced Jurakt cells. Panel E and G: CXCR4 staining of induced Jurkat cells. The fluorescent cell in panel G is representative of a minor population of cells in the induced culture (<5%) with a CXCR4 surface distribution similar to uninduced cells. Panels B, D, F and H show phase contrast images of the same fields of cells shown in left panels.