Figure 5
From: Genetic diversity and silencing suppression effects of Rice yellow mottle virus and the P1 protein
Effect of the P1 protein from different RYMV isolates on silencing suppression in rice. Transgenic L10 leaves were analysed after different treatments; non-biolistic delivery (ND), buffer delivery (BD), biolistic delivery with a empty 35S vector (35S), or vectors containing different sP1 from RYMV isolates representative of the viral phylogeny (CI63, Mg1, Tz8, Tz3, BF1). Non transgenic Tai and transgenic L4 served as controls. (A) Photographs correspond to GUS staining at 2 dpd of inoculated leaves. (B) Quantitative effect of different P1 at 2 dpd on gus-specific siRNA with Northern blot experiments. EtBr staining of rRNA served as a loading control.