Figure 2From: Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell cultureSouthern blotting analysis of pOka-BAC and pOka-BACΔ58. DNA. Purified pOka-BAC DNA and pOka-BACΔ58 DNA were digested by Bam HI and loaded onto a 0.5% TBE agarose gel. The fragments recognized by the ORF58, ORF62/71 and Kmr probes (right) are indicated by arrowheads in the photograph (left). Southern blotting was performed using ORF58, ORF62/71, or the Kmr gene as a probe.Back to article page