Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Dissecting the role of putative CD81 binding regions of E2 in mediating HCV entry: Putative CD81 binding region 1 is not involved in CD81 binding

Figure 2

Alanine substitutions within putative CD81 binding regions dramatically affect HCVpp entry. 293T cells were cotransfected with the HIV-luc packaging vector along with HCV E1E2 mutant expression plasmids. HCVpp was harvested at 24 h post-transfection and used to infect susceptible cell lines (A) Huh7 and (B) Hep3B. Infectivity was measured 72 h pi using a luciferase reporter assay. Infectivity of each mutant is expressed as a percentage of the infectivity observed for the wild-type (wt) H77 HCV E1E2. Values shown are the mean and standard error for a minimum of three assays.

Back to article page

Virology Journal

ISSN: 1743-422X