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Table 1 Relative occupancy of RNA Polymerase II on the SV40 genome after treatment with HDACi.

From: HDAC inhibitors stimulate viral transcription by multiple mechanisms

  

30 Min

8 Hour

48 Hour

EARLY

Untreated

63 ± 1.5

43 ± 1.5

42 ± 2.0

 

NaBu Treated

72 ± 2.0

67 ± 1.0*

54 ± 2.5

 

TSA Treated

78 ± 1.5

65 ± 2.0*

57 ± 1.0

LATE

Untreated

34 ± 2.0

51 ± 3.0

52 ± 2.5

 

NaBu Treated

40 ± 1.0

58 ± 2.0

84 ± 1.0**

 

TSA Treated

39 ± 1.5

64 ± 1.0

89 ± 2.5**

PROMOTER

Untreated

51 ± 1.5

28 ± 3.5

60 ± 0.5

 

NaBu Treated

53 ± 2.0

51 ± 1.0*

62 ± 3.0

 

TSA Treated

57 ± 1.0

59 ± 2.5*

67 ± 1.5

  1. **P < 0.001; * P < 0.05 (Student's t test). Untreated SV40 minichromosomes or SV40 minichromosomes treated with 250 μM NaBu or 120 nM TSA were isolated from cells infected with 776 wild-type virus for 48 hours. Purified SV40 minichromosomes were immunoprecipitated with 10 μl antibody to RNA polymerase II using the ISF technique and PCR amplified using primer sets to the early, late and promoter regions. Data in the table is represented as Mean ± S.E (n = 5 for treated samples and n = 3 for untreated control samples).
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Virology Journal

ISSN: 1743-422X