Skip to main content

Table 1 Relative occupancy of RNA Polymerase II on the SV40 genome after treatment with HDACi.

From: HDAC inhibitors stimulate viral transcription by multiple mechanisms

   30 Min 8 Hour 48 Hour
EARLY Untreated 63 ± 1.5 43 ± 1.5 42 ± 2.0
  NaBu Treated 72 ± 2.0 67 ± 1.0* 54 ± 2.5
  TSA Treated 78 ± 1.5 65 ± 2.0* 57 ± 1.0
LATE Untreated 34 ± 2.0 51 ± 3.0 52 ± 2.5
  NaBu Treated 40 ± 1.0 58 ± 2.0 84 ± 1.0**
  TSA Treated 39 ± 1.5 64 ± 1.0 89 ± 2.5**
PROMOTER Untreated 51 ± 1.5 28 ± 3.5 60 ± 0.5
  NaBu Treated 53 ± 2.0 51 ± 1.0* 62 ± 3.0
  TSA Treated 57 ± 1.0 59 ± 2.5* 67 ± 1.5
  1. **P < 0.001; * P < 0.05 (Student's t test). Untreated SV40 minichromosomes or SV40 minichromosomes treated with 250 μM NaBu or 120 nM TSA were isolated from cells infected with 776 wild-type virus for 48 hours. Purified SV40 minichromosomes were immunoprecipitated with 10 μl antibody to RNA polymerase II using the ISF technique and PCR amplified using primer sets to the early, late and promoter regions. Data in the table is represented as Mean ± S.E (n = 5 for treated samples and n = 3 for untreated control samples).