Figure 3
From: HDAC inhibitors stimulate viral transcription by multiple mechanisms
Effects of HDACi treatment on SV40 Replication. (a) BSC-1 cells infected for 48 hours with wild type 776 SV40 virus were either untreated or treated with 250 μM NaBu or 120 nM TSA and radiolabeled with methyl-[3H] thymidine. SV40 DNA was isolated and methyl-[3H] thymidine incorporation into newly replicating DNA was counted in a liquid scintillation analyzer and is represented as the percentage of methyl-[3H] thymidine incorporation relative to untreated cells. Data represented in the graph is Mean ± S.E. (n = 6). (b) 48 hour SV40 minichromosomes either untreated or treated with 250 μM NaBu or 120 nM TSA were immune selected using 10 μl of antibody against RPA70. Data is expressed as percentage of SV40 minichromosomes containing RPA70 and represent the average of three independent experiments.