Figure 1

Effects of HDACi treatment on SV40 transcription. (a) Total protein extracted from 30 min and 48 hour SV40 infected BSC-1 cells and either untreated or treated with 250 μM NaBu or 120 nM TSA were separated using SDS PAGE followed by western blot analysis using antibody against T antigen (for 30 mins), VP1 (for 48 hours) and GAPDH (for both 30 mins and 48 hours). (b) Total RNA extracted from 30 min, 8 hour and 48 hour SV40 infected BSC-1 cells untreated and treated with 250 μM NaBu or 120 nM TSA were reverse transcribed and the amount of mRNA for T antigen and VP1 were analyzed using real time PCR using primer sets against early the coding region and late coding region, respectively. Data is expressed as the fold increase in expression levels compared to the mRNA from untreated SV40 infected BSC-1 cells and represent the average of three independent experiments.