Skip to main content

Table 2 Summary of RT-PCR detection of TULV S and M segment RNA.

From: Tula hantavirus isolate with the full-length ORF for nonstructural protein NSs survives for more consequent passages in interferon-competent cells than the isolate having truncated NSs ORF

    Passages
Cells Infection with TULV isolates TULV isolate 1 2 3 4 5 6 7 8 9 10
Vero E6 Lodz & Moravia Lodz + + Xa + + + + + + +
   Moravia + + X + + + + + + +
MRC5 Lodz & Moravia Lodz + + + - - - NDb ND ND ND
   Moravia + - - - - - ND ND ND ND
MRC5 1st passage from VeroE6 Lodz + + + + + - ND ND ND ND
   Moravia + + + +/- c - - ND ND ND ND
  1. aRNA pellet from passage 3 was lost and therefore we were unable to detect viruses in this passage;
  2. bND = not done;
  3. cThe S-specific RT-PCR was positive up to passage 4; the M-specific RT-PCR was positive up to passage 3.