Tula hantavirus isolate with the full-length ORF for nonstructural protein NSs survives for more consequent passages in interferon-competent cells than the isolate having truncated NSs ORF

Table 2 Summary of RT-PCR detection of TULV S and M segment RNA.

			Passages
Cells	Infection with TULV isolates	TULV isolate	1	2	3	4	5	6	7	8	9	10
Vero E6	Lodz & Moravia	Lodz	+	+	X^a	+	+	+	+	+	+	+
		Moravia	+	+	X	+	+	+	+	+	+	+
MRC5	Lodz & Moravia	Lodz	+	+	+	-	-	-	ND^b	ND	ND	ND
		Moravia	+	-	-	-	-	-	ND	ND	ND	ND
MRC5	1st passage from VeroE6	Lodz	+	+	+	+	+	-	ND	ND	ND	ND
		Moravia	+	+	+	+/- ^c	-	-	ND	ND	ND	ND

^aRNA pellet from passage 3 was lost and therefore we were unable to detect viruses in this passage;
^bND = not done;
^cThe S-specific RT-PCR was positive up to passage 4; the M-specific RT-PCR was positive up to passage 3.

ISSN: 1743-422X