La Crosse virus infectivity, pathogenesis, and immunogenicity in mice and monkeys

Table 1 Spread of virus from brain to spinal cord after IP inoculation with 10^4.5 PFU LACV/human/1960.

	No. of mice with detectable viral antigen or histopathologic lesions^a
	Brain										Spinal cord
	Olfactory bulb		Thalamus		Cerebral cortex		Medulla		Cerebellum		Cervical		Thoracic		Lumbar
Day	IHC^b	HE^c	IHC	HE	IHC	HE	IHC	HE	IHC	HE	IHC	HE	IHC	HE	IHC	HE
1	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
2	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
3	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
4	1	1	1	1	1	0	1	1	0	0	0	0	0	0	0	0
5	3	0	3	1	2	0	1	0	1	0	1	1	1	1	1	1
6	3	2	3	3	3	2	3	2	1	0	2	2	2	1	1	0

^a Data displayed as total positive by IHC and HE (n = 3). Viral antigen or lesions were not observed in nasal turbinates, muscle, spleen, or pancreas. Tissues from three control mice were collected on days 1 and 6 and processed for HE and IHC comparisons, viral antigen or lesions were not present.
^b Immunohistochemical stain
^c Hemotoxylin and eosin stain

ISSN: 1743-422X