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Table 1 Degenerate oligonucleotides for PCR amplification of VL and VH of mAb 54.6

From: Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands

MVH-R 5'- GAC HGA TGG GGS TGT YGT GCT AGC TGN RGA GAC DGT GA -3'
MVK-R 5'- GGA TAC AGT TGG TGC AGT CGA CTT ACG TTT KAT TTC CAR CTT -3'
VK1-F 5'- GGG GAT ATC CAC CAT GGA GAC AGA CAC ACT CCT GCT AT -3'
VK2-F 5'- GGG GAT ATC CAC CAT GGA TTT TCA AGT GCA GAT TTT CAG -3'
VK3-F 5'- GGG GAT ATC CAC CAT GGA GWC ACA KWC TCA GGT CTT TRT A -3'
VK4-F 5'- GGG GAT ATC CAC CAT GKC CCC WRC TCA GYT YCT KGT -3'
VK5-F 5'- GGG GAT ATC CAC CAT GAA GTT GCC TGT TAG GCT GTT G -3'
VH1-F 5'- GGG GAT ATC CAC CAT GGR ATG SAG CTG KGT MAT SCT CTT -3'
VH2-F 5'- GGG GAT ATC CAC CAT GRA CTT CGG GYT GAG CTK GGT TTT -3'
VH3-F 5'- GGG GAT ATC CAC CAT GGC TGT CTT GGG GCT GCT CTT CT -3'
VH4-F 5'- GGG GAT ATC CAC CAT GAT RGT GTT RAG TCT TYT GTR CCT G -3'