Table 1 Degenerate oligonucleotides for PCR amplification of V_L and V_H of mAb 54.6

MVH-R

5'- GAC HGA TGG GGS TGT YGT GCT AGC TGN RGA GAC DGT GA -3'

MVK-R

5'- GGA TAC AGT TGG TGC AGT CGA CTT ACG TTT KAT TTC CAR CTT -3'

VK1-F

5'- GGG GAT ATC CAC CAT GGA GAC AGA CAC ACT CCT GCT AT -3'

VK2-F

5'- GGG GAT ATC CAC CAT GGA TTT TCA AGT GCA GAT TTT CAG -3'

VK3-F

5'- GGG GAT ATC CAC CAT GGA GWC ACA KWC TCA GGT CTT TRT A -3'

VK4-F

5'- GGG GAT ATC CAC CAT GKC CCC WRC TCA GYT YCT KGT -3'

VK5-F

5'- GGG GAT ATC CAC CAT GAA GTT GCC TGT TAG GCT GTT G -3'

VH1-F

5'- GGG GAT ATC CAC CAT GGR ATG SAG CTG KGT MAT SCT CTT -3'

VH2-F

5'- GGG GAT ATC CAC CAT GRA CTT CGG GYT GAG CTK GGT TTT -3'

VH3-F

5'- GGG GAT ATC CAC CAT GGC TGT CTT GGG GCT GCT CTT CT -3'

VH4-F

5'- GGG GAT ATC CAC CAT GAT RGT GTT RAG TCT TYT GTR CCT G -3'