Table 1 Oligonucleotide primers used for amplification of LASV genes expressed in mammalian cells (see Additional file 1)
LASV gene amplified | LASV primer | Oligonucleotide primer sequence |
|---|---|---|
GPC | 5' GPC | GTAGCTAGC ATGGGACAAATAGTGACATTCTTCCAG |
| Â | 3' GPC | GGTACCAAGCTT TCA GTCA TCTCTTCCATTTCACAGGCAC |
GPC-FLAG | 5' GPC | Â |
| Â | 3' GPC-FLAG | CGATAAGCTT TCA GTCA GCCCTTGTCGTCGTCGTCCTTGTAGTC TCTCTTCCATTTCACAGGCAC |
sGP1 | 5' GPC | Â |
| Â | 3' sGP1 | GGTACCAAGCTT TCA GTCA TAGCAATCTTCTACTAATATAAATATCTCT |
sGP1-FLAG | 5' GPC | Â |
| Â | 3' sGP1-FLAG | CGATAAGCTT TCA GTCA GCCCTTGTCGTCGTCGTCCTTGTAGTC TAGCAATCTTCTACTAATATA |
sGP1-hHC | 5' hHC-sGP1 | GATCGCTAGC GCCGCCACCATGGGCTGGAGCTGCATCATCCTGTTCCTGGTGGCCACCGCCACCGGCGTGCACAGC ACCAGTCTTTATAAAGGGGTT |
| Â | 3' sGP1 | Â |
sGP1-hλLC | 5' hλLC-sGP1 | AAGCTGGCTAGC CACCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG ACCAGTCTTTATAAAGGGGTT |
| Â | 3' sGP1 | Â |
GP1-TM | 5' GPC | Â |
| Â | 3' GP1-TM | GGTACCAAGCTT TCA GTCA TGGTATTTTGACTAGGTGAAGGAAGATGCTAATAAGATAGAAACTTGTGCTGAACACAAAGAGGTCAACTAGACCCAATGG TAGCAATCTTCTACTAATATAAATATCTCT |
sGP2-GPC SP | 5' GPC | Â |
| Â | sGP2-GPC SP | CAGTGTCCATGTGAATGTGCC GGTTGTGCAAGACCATCCACACAA |
| Â | 5' GPC | Â |
| Â | 3' GP2preTM | GGTACCAAGCTT TCA GCTA TGTCTTCCCCTGCCTCTCCAT |
sGP2-hHC | 5' hHC-sGP2 | GATCGCTAGC GCCGCCACCATGGGCTGGAGCTGCATCATCCTGTTCCTGGTGGCCACCGCCACCGGCGTGCACAGC GGCACATTCACATGGACACTG |
| Â | 3' sGP2preTM | Â |
sGP2-hHC-TM | 5' hHC-sGP2 | Â |
| Â | 3' GP2-TM | GGTACCAAGCTT TCA GTCA TGGTATTTTGACTAGGTGAAGGAA |
sGP2-hHC-FLAG | 5' hHC-sGP2 | Â |
| Â | 3' GP2preTM-FLAG | CGATAAGCTT TCA GTCA GCCCTTGTCGTCGTCGTCCTTGTAGTC TGTCTTCCCCTGCCTCTCCAT |
GP2-hHC-TM-FLAG | 5' hHC-sGP2 | Â |
| Â | 3' GP2-TM-FLAG | CGATAAGCTT TCA GTCA GCCCTTGTCGTCGTCGTCCTTGTAGTC TGGTATTTTGACTAGGTGAAGGAA |
s GP2-hλLC | 5' hλLC-sGP2 | AAGCTGGCTAGC CACCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG GGCACATTCACATGGACACTG |
| Â | 3' sGP2preTM | Â |
sGP2-hλLC-TM | 5' hλLC-sGP2 |  |
| Â | 3' GP2-TM | Â |
GPCΔTMΔIC | 5' GPC |  |
| Â | 3' GP2preTM | Â |
GPCΔTMΔIC-FLAG | 5' GPC |  |
| Â | 3' GP2preTM-FLAG | CGATAAGCTT TCA GTCA GCCCTTGTCGTCGTCGTCCTTGTAGTC TGTCTTCCCCTGCCTCTCCAT |
GPCΔTM-FLAG | 5' GP2 TM deletion | AAAATACCAACTCATAGGCATATTGTAG |
| Â | 3' TM deletion | GGAGTATATGGAGAGGCAGGGGAAGACA |
GPCΔ59–249ΔTMΔIC | 5'GPC |  |
| Â | 3' SKI-1/GP2 | CAGTGTCCATGTGAATGTGCC TAGCAATCTTCTACTAATATAAATATCTCT GGTTGTGCAAGACCTACCACACAA |
| Â | 5' GPC | Â |
| Â | 3' GP2preTM | Â |
GPCΔ59–249ΔTMΔIC-FLAG | 5'GPC |  |
| Â | 3' SKI-1/GP2 | Â |
| Â | 5' GPC | Â |
| Â | 3' GP2preTM-FLAG | Â |
GPCΔ59–249ΔTM | 5' GPC |  |
| Â | 3' SKI-1/GP2 | Â |
| Â | 5' GPC | Â |
| Â | 3' GPC-FLAG | Â |