Detection of WSSV403 transcript in shrimp. (A) WSSV403 transcription was detected in shrimp during WSSV infection by time course RT-PCR. WSSV403 transcription was detected in shrimp before WSSV inoculation. (B) WSSV403 transcript was found in SPF shrimp RNA by nested RT-PCR. Three latency-associated-genes, WSSV151, 366 and 427, were indicated as positive controls, while VP19 and VP28 were used as negative controls. (C) Amplification profiles and dissociation curves of WSSV403 in real time PCR using WSSV DNA, total RNA from WSSV-infected shrimp and amplified RNA from SPF shrimp. Water was used as a negative control.