Table 1 Primers for real-time PCR.
| Primer |
Sequence (5'> 3') | |
|---|---|---|
| TLR2 | Forward | CAGGGCTCACAGAAGCTGTAA |
| Reverse | GCCCAGGGAAGAAAAAGAATC | |
| TLR3 | Forward | TAGCAGTCATCCAACAGAATCAT |
| Reverse | AATCTTCTGAGTTGATTATGGGTAA | |
| TLR4 | Forward | ACACAGAAGAGCTGGCATGA |
| Reverse | GGTTGTCGGGGATTTTGTAG | |
| TLR9 | Forward | CTTCCCTGTAGCTGCTGTCC |
| Reverse | CCTGCACCAGGAGAGACAG | |
| IFN-α(1/13) | Forward | TGGCTGTGAAGAAATACTTCCG |
| Reverse | TGTTTTCATGTTGGACCAGATG | |
| IFN-β | Forward | TCTCCACGACAGCTCTTTCCA |
| Reverse | ACACTGACAATTGCTGCTTCTTTG | |
| MyD88 | Forward | TGGCACCTGTGTCTGGTCTA |
| Reverse | ACATTCCTTGCTCTGCAGGT | |
| IRF-3 | Forward | GTTCTGTGTGGGGGAGTCAT |
| Reverse | CTGTTGGAAATGTGCAGGTC | |
| TRIF | Forward | CCTCCTCCTCCTCCTTCATC |
| Reverse | GCGTGGAGGATCACAAAGTT |