Table 1 Primers for real-time PCR.
Primer | Â | Sequence (5'> 3') |
|---|---|---|
TLR2 | Forward | CAGGGCTCACAGAAGCTGTAA |
| Â | Reverse | GCCCAGGGAAGAAAAAGAATC |
TLR3 | Forward | TAGCAGTCATCCAACAGAATCAT |
| Â | Reverse | AATCTTCTGAGTTGATTATGGGTAA |
TLR4 | Forward | ACACAGAAGAGCTGGCATGA |
| Â | Reverse | GGTTGTCGGGGATTTTGTAG |
TLR9 | Forward | CTTCCCTGTAGCTGCTGTCC |
| Â | Reverse | CCTGCACCAGGAGAGACAG |
IFN-α(1/13) | Forward | TGGCTGTGAAGAAATACTTCCG |
| Â | Reverse | TGTTTTCATGTTGGACCAGATG |
IFN-β | Forward | TCTCCACGACAGCTCTTTCCA |
| Â | Reverse | ACACTGACAATTGCTGCTTCTTTG |
MyD88 | Forward | TGGCACCTGTGTCTGGTCTA |
| Â | Reverse | ACATTCCTTGCTCTGCAGGT |
IRF-3 | Forward | GTTCTGTGTGGGGGAGTCAT |
| Â | Reverse | CTGTTGGAAATGTGCAGGTC |
TRIF | Forward | CCTCCTCCTCCTCCTTCATC |
| Â | Reverse | GCGTGGAGGATCACAAAGTT |