Figure 2
From: The YPLGVG sequence of the Nipah virus matrix protein is required for budding
NiV M sequence required for budding and possible late domain element. (A) NiV M mutants were constructed with single alanine substitution mutations in the YPLGVG sequence (underlined), or with the whole sequence deleted (dashes). (B) Wild-type NiV M and the mutants depicted in A were all expressed in cells and released protein was pelleted through a 20% sucrose cushion. Proteins derived from the cell lysate (L) or culture supernatant (S) were immunoprecipitated with MAb F45G5 and analyzed by SDS-PAGE followed by autoradiography. (C) Wild-type and mutant P93A HeV M were expressed in cells and a budding assay was performed as described in the Methods.