Figure 1
From: The YPLGVG sequence of the Nipah virus matrix protein is required for budding
Site-directed proline mutagenesis. (A) ClustalW alignment of the M proteins from Nipah virus (NiV), Hendra virus (HeV), Tupaia paramyxovirus (TPMV), measles virus (MeV), Sendai virus (SeV), Newcastle disease virus (NDV), and simian virus 5 (SV5). Sequences of interest are boxed. Alignment was performed as described in the Methods. (B) Known L-domains are shown along with corresponding hypothetical L-domain sequences of NiV M (boxed in A). Underlined proline residues were mutated to alanine. (C) Mutant NiV M proteins, along with wild-type, were expressed in cells and released protein was pelleted through 10% sucrose. Proteins derived from the cell lysate (L) or culture supernatant (S) were immunoprecipitated using MAb F45G5 and analyzed by SDS-PAGE followed by autoradiography as described in the Methods.